diff --git a/src/slic3r/GUI/Plater.cpp b/src/slic3r/GUI/Plater.cpp
index 4526bc51d..cb7066630 100644
--- a/src/slic3r/GUI/Plater.cpp
+++ b/src/slic3r/GUI/Plater.cpp
@@ -3558,6 +3558,14 @@ void Plater::priv::on_select_preset(wxCommandEvent &evt)
      */
         wxGetApp().obj_list()->update_object_list_by_printer_technology();
     }
+
+#ifdef __WXMSW__
+    // From the Win 2004 preset combobox lose a focus after change the preset selection
+    // and that is why the up/down arrow doesn't work properly
+    // (see https://github.com/prusa3d/PrusaSlicer/issues/5531 ).
+    // So, set the focus to the combobox explicitly
+    combo->SetFocus();
+#endif
 }
 
 void Plater::priv::on_slicing_update(SlicingStatusEvent &evt)
diff --git a/src/slic3r/GUI/PresetComboBoxes.cpp b/src/slic3r/GUI/PresetComboBoxes.cpp
index 2ef7b3e1f..6031edf78 100644
--- a/src/slic3r/GUI/PresetComboBoxes.cpp
+++ b/src/slic3r/GUI/PresetComboBoxes.cpp
@@ -905,6 +905,13 @@ TabPresetComboBox::TabPresetComboBox(wxWindow* parent, Preset::Type preset_type)
         }
 
         evt.StopPropagation();
+#ifdef __WXMSW__
+        // From the Win 2004 preset combobox lose a focus after change the preset selection
+        // and that is why the up/down arrow doesn't work properly
+        // (see https://github.com/prusa3d/PrusaSlicer/issues/5531 ).
+        // So, set the focus to the combobox explicitly
+        this->SetFocus();
+#endif    
     });
 }